用户名: 密 码:
第三方登录:
数据库:研究论文另存文本打印浏览 【字体放大】 【字体还原】
【题目】IIsolation,Culture and Biological Characteristics of Tibetan Mastiff (Canis lupus Familiaris) Muscle Satellite Cells
【作者】关伟军
【摘要】
  With the ability of self-renewal and multipotenntial differentiation, muscle satellite cells (MSCs) are recognized as a population of tissue-specific progenitors which play an important role in the growth,repair and regeneration of muscles. Satellite cells have been isolated for a very long time,but the techniques of isolation are still to be improved.In this study,MSCs were obtained from aborted Tibetan mastiff fetuses (about 60 days) under sterile conditions.Primary MSCs were sub-cultured to passage 25 in vitro. The gene of C-met, MyoD1,Desmin and Pax7 were detected by RT-polymerase chain reaction and immunofluorescence assays. The result showed that they were positive in MSCs of Tibetan mastiff.The growth of different passages cells typically appeared in S curve.Furthermore,myogenic,osteogenic and adipogenic differenciation of MSCs were successfully induced.The results denoted that the MSCs obtained from Tibetan Mastiffs exhibited the characteristics of multipotent stem cells.Therefore,this work provided a theoretical and technical basis for Tibetan mastiff,even its genetic resources preservation and laid a solid foundation for studying the mechanism of skeletal muscle growth and development in future.
【关键词】Tibetan Mastiff,Muscle Satellite Cells,Identification,Pluripotent,Differentiation
【资料来源】Journal of Biomaterials and Tissue Engineering
【全文浏览】全文浏览



copyright © 2019 家养动物种质资源库
版权所有:中国农业科学院北京畜牧兽医研究所
维护制作:北京畜牧兽医研究所智慧畜牧创新团队